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What is the composition of the column?

Beijing Zhongyi Yusheng Technology Co., Ltd.

The column is a column tube filled with a stationary phase for separating mixed components, generally made of metal or glass, and has a straight tube type, a coil type, a U-shaped tube, etc., and is widely used in various fields. . The column is mainly composed of those components? The following is a detailed description of the components of the column, I hope to help everyone.

Column components

Chromatography is a means of separation and analysis. Separation is the core, so the column responsible for separation is the heart of the chromatographic system. The requirements for the column are high column efficiency, good selectivity, and fast analysis speed. Commercially available for H

PLC

Various particulate fillers such as porous silica gel and silica-based bonded phase, alumina, organic polymer microspheres (including ion exchange resins), porous carbon, etc., generally have a particle size of 3, 5, 7, 10 μm, etc. The theoretical value of the column efficiency can reach 50,000 to 160,000 / m. For the general analysis, only 5000 columns of column efficiency is needed; for homologue analysis, only 500 can be used; for difficult to separate substances, up to 20,000 columns can be used, so the column length of 10~30cm is generally Meet the needs of complex mixture analysis.

The column consists of a column tube, a pressure cap, a ferrule (seal ring), a sieve plate (filter), a joint, a screw, and the like. The column tube is mostly made of stainless steel. When the pressure is not higher than 70 kg/cm2, thick-walled glass or quartz tube can also be used. The inner wall of the tube requires a high degree of smoothness. In order to improve the efficiency of the column and reduce the wall effect, the inner wall of the stainless steel column is polished. Some people also apply fluoroplastic on the inner wall of the stainless steel column to improve the smoothness of the inner wall, and the effect is the same as polishing. Also used is a fused silica or glass lining for thin pipe columns. The column joints at both ends of the column are equipped with sieve plates, which are sintered stainless steel or titanium alloy with a pore size of 0.2~20&m.

Ic

Ro;m (5~10μm), depending on the particle size of the filler, is intended to prevent the filler from leaking out.

According to the application, the column can be divided into two types: analytical type and preparative type. The size and specifications are also different: 1 conventional analytical column (constant column), inner diameter 2~5mm (commonly used 4.6mm, domestic 4mm and 5mm), column length 10~30cm 2 narrow bore (also known as thin bore, semi-microcolumn), inner diameter 1~2mm, column length 10~20cm; 3 capillary column (also known as microcolumn microcolumn), inner diameter 0.2~0.5 Mm; 4 semi-preparative column, inner diameter> 5mm; 5 laboratory preparation column, inner diameter 20~40mm, column length 10~30cm; 6 production preparation column inner diameter up to several tens of centimeters. The inner diameter of the column is generally determined by the length of the column, the particle size of the filler, and the flow rate of the fracture, in order to avoid the wall effect.

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Beijing Zhongyi Yusheng Technology Co., Ltd.